proH [2017-8-11 16:0:3]

pyrroline-5-carboxylate reductase

locus: BSU18480

pI: 7.75

mw: 31.88 kDa

protein length: 297 aa

gene length: 891 bp

function: osmoadaptive de novo production of proline

product: pyrroline-5-carboxylate reductase

essential: no

ec: 1.5.1.2

synonyms: yoxE

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.2|Biosynthesis/ acquisition of proline]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.6|Coping with hyper-osmotic stress]

Gene

Coordinates: 2,016,845 → 2,017,738

The protein

Catalyzed reaction/ biological activity

• 1-pyrroline-5-carboxylate + NADPH + H⁺ --> L-proline + NADP⁺ [pubmed|28824574]

Protein family

• [SW|pyrroline-5-carboxylate reductase family] [pubmed|28824574]

Paralogous protein(s)

• [protein|93000B579F630E9F0C6EED40D6661E5E15141FDE|ProI]

[SW|Cofactors]

• NADPH [pubmed|28824574]

Effectors of protein activity

• feedback-inhibited by proline [pubmed|28824574]

Structure

• [PDB|5BSE] (from ''Medicago truncatula'', 36% identity) [pubmed|26579138]

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|542871C77BE841AA59A65FFE8A44AB85BF9180F0|proH]-[gene|3F47513FB71276EF1E99B8C427DC8DC0786F8C19|proJ]
  description: [Pubmed|21296969]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|21784929], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulation

  • expressed under conditions of osmotic stress [Pubmed|21784929]

Biological materials

Mutant

• MGNA-B083 (proH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1082 NBRP B. subtilis, Japan]

References

Reviews

• 25367752

Original publications

• 11418582,21296969,25344233,21784929,27766092,28752945,28824574,26579138